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932 Part VII: Neutrophils, Eosinophils, Basophils, and Mast Cells Chapter 60: Structure and Composition of Neutrophils, Eosinophils, and Basophils 933

TABLE 60–2. Glycolytic and Related Enzyme Activities in Neutrophils
Activity at 37°C in Activity at 30°C in Activity at 25°C in Mixed
Enzyme Neutrophils* Neutrophils † Leukocytes ‡
Hexokinase 78 ± 14 39.6 ± 27.3 —
Phosphofructokinase 36 ±2 — —
Aldolase 76 ± 7 118.7 ± 27.4 123
Glucosephosphate isomerase 4930 ± 716 — —
Triosephosphate isomerase 7853 ± 323 — 2189
Glyceraldehyde dehydrogenase 3683 ± 124 — 242
Monophosphoglycerate mutase 508 ± 35 — —
Phosphoglycerate kinase 3744 ± 197 — 890
Enolase 136 ± 17 — 734
Pyruvate kinase 173 ± 11 4125 ± 549 976
Lactate dehydrogenase 1128 ± 51 2981 ± 893 1165
Glucose-6-phosphate dehydrogenase 517 ± 11 596 ± 116.6 176
6-Phosphogluconate dehydrogenase 287 ± 5 — —
Glutathione reductase 63 ± 7 — —
Glutathione peroxidase 17 ± 3 — —
Glutamic oxaloacetic transaminase 25 ± 2 — 43
Adenylate kinase 32 ± 2 163 ± 9.9 149
α-Glycerophosphate dehydrogenase — — 23
Isocitric dehydrogenase — — 47
Fructose 1,6-diphosphatase — 0.76 ± 0.18 —
Isocitrate dehydrogenase — 44.1 ± 6.4 —
Citrate synthase — 32.0 ± 5.4 —
Malate dehydrogenase — 482 ± 62.6 —
Transketolase — 0.99 ± 0.27 —
Phosphorylase A — 9.60 ± 2.66 —
Lipoamide dehydrogenase — 29.7 ± 13.8 —
Ca ATPase — — 28
2+
Mg ATPase — — 30
2+
ATPase, adenosine triphosphatase.
*IU/mg protein. IU/L. Calculated as units/10 leukocytes, assuming a protein content of 7.4 mg/10 leukocytes.
†
‡
11
11
TABLE 60–3. Proteins Synthesized by Neutrophils
Cytokines Receptors Chemokines Growth Factors Miscellaneous
TNF-α IL-1 receptor antagonist (IL-1RA) IL-8 G-CSF Fas ligand
IL-1β GRO-α M-CSF CD40
IL-12 TGF-β GRO-β GM-CSF CD83
IFN-α IP-10 IL-3 CCR6
IL-6 MIP-1α VEGF CCR2
Oncostatin M MIP-1β TGF-β HLA-DR
MCP-1
CCR, C-C chemokine receptor; CD, cluster of differentiation; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-monocyte col-
ony-stimulating factor; GRO, growth-related oncogene; HLA-DR, human leukocyte antigen-D–related; IFN, interferon; IL, interleukin; IP, inter-
feron-inducible protein; MCP, membrane cofactor protein; M-CSF, monocyte colony-stimulating factor; MIP, macrophage inflammatory protein;
TGF, transforming growth factor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor.

Kaushansky_chapter 60_p0923-0938.indd 933 9/18/15 10:35 PM