Page 600 - Clinical Hematology_ Theory _ Procedures ( PDFDrive )
P. 600

■    Name several conditions that can produce a high total leukocyte                                        ■    List and describe the types of crystals that may be observed in syno-
                   count in peritoneal   uid.                                                                                  vial   uid.

                 List the types of cells that can be seen in peritoneal effusion and                                         Associate various types of crystals with particular disorders.

                   associate these cell types with a representative disorder.                                                De  ne birefringence and explain its use in the identi  cation of crys-


               ■    Describe the anatomy of the pericardium.                                                                   tals in body   uids.
                 Associate the various types and causes of pericardial effusion.                                             Describe the normal total cell count and differential in synovial   uid.


               ■    Name a cause of an increased total leukocyte count with mostly                                         ■    Compare the laboratory   ndings in nonin  ammatory and in  amma-
                   polymorphonuclear segmented neutrophils (PMNs).                                                             tory arthritis.




               Seminal   uid                                                                                               Body   uid slide preparation


               ■    Describe the anatomical structures and their respective cellular                                       ■    Compare the features of various methods of body   uid sediment
                   and/or chemical components.                                                                                 preparation.

                 Describe health, medical, and social behaviors that can negatively                                        ■    Differentiate  the  characteristics  of  Wright-Giemsa  and

                   in  uence the sperm count or other seminal analysis results.                                                Papanicolaou’s stains.


               ■    Discuss the proper collection and handling of seminal   uid.                                           Amniotic   uid

               ■    Name the normal number of sperm cells per milliliter or per liter.
                                                                                                                           ■    Describe the composition of amniotic   uid and its importance to
               ■    Name the types of microscopic assays and the respective normal                                             the fetus.
                   values.
                                                                                                                           ■    Interpret automated lamellar body counts in amniotic   uid.


               Synovial   uid                                                                                              Case studies


               ■    De  ne the term arthrocentesis.                                                                          Analyze the patient history, clinical signs and symptoms, and labo-

               ■    List at least three disorders that can be diagnosed de  nitively by                                        ratory data for the stated case studies, answer the related critical
                   synovial   uid analysis.                                                                                    thinking questions, and conclude the most likely diagnosis.


               ■    List several sites that may be aspirated.

               ■    Name the tests that should be included in the routine analysis of                                         NOTE:
                   synovial   uid.                                                                                                indicates MLT and MLS core content


               ■    Name the procedures included in the gross examination of synovial                                         ■
                                                                                                                                indicates MLT (optional) and MLS advanced content
                    uid.













               INTRODUCTION

                                                                                                                                TABLE        29.1       Body Fluids
               Frequently,  the  analysis  o   body  f uids  is  assigned  to  the

               hematology  laboratory.  Gross  physical  examination,  total                                                   Fluids                                            Synonyms

               cell count, microscopic examination, and other special tests

               are  generally  within  the  job  responsibility  o   hematology                                                Bronchoalveolar lavage                            Bronchial washings

               technicians and technologists. Because clinical correlations                                                    Cerebrospinal   uid                               Spinal   uid

               o  body f uid analyses are diagnostically important, clinical

               in ormation is presented in each section o  this chapter.                                                                                                         Lumbar puncture   uid

                    Chemical analyses and microbial and cytological exami-                                                                                                       Ventricular   uid

               nations are generally per ormed in the chemistry, microbiol-                                                                                                      Meningeal   uid

               ogy, and cytology departments, respectively. For this reason,                                                   Synovial   uid                                    Joint   uid

               speci  c procedures in these disciplines are not included in

               this chapter.                                                                                                   Peritoneal                                        Dialysate   uid

                    Sterile body f uid can be  ound in various body cavities                                                   (abdominal)   uid                                 Paracentesis   uid

               under normal conditions. In diverse disorders and disease                                                                                                         Ascitic   uid

               processes, the quantity o  these f uids can increase signi  -                                                   Pericardial   uid                                 Fluid from around the

               cantly. Fluid specimens aspirated  rom di  erent anatomical                                                                                                       heart

               sites (  able 29.1) can be analyzed  or the total number o

               cells, di  erentiation o  cell types, chemical composition, and                                                                                                   Pericardiocentesis   uid

               microbial contents. All body f uids should be handled with                                                      Pleural   uid                                     Chest   uid

               caution. Standard precautions must be practiced.                                                                                                                  Thoracic   uid

                    T e  type  o   examination  per ormed  on  the  body  f uid                                                                                                  Thoracentesis   uid

               depends on the source o  the specimen. However, a portion

               o  the examination o  cerebrospinal   uid (CSF); serous f uids                                                  Seminal   uid                                     Semen
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